201545

Fast Blue RR

• Synonym(e): 4-Benzoylamino-2,5-dimethoxyanilin, Azoic Diazo No. 24
• Empirische Formel (Hill-System): C₁₅H₁₆N₂O₃
• CAS-Nummer: 6268-05-9
• Molekulargewicht: 272.30
• PubChem Substance ID: 24852010
• UNSPSC Code: 12171500
• Colour Index Number: 37155
• NACRES: NA.47
• EC Number: 228-441-6
• MDL number: MFCD00008369

Eigenschaften

• assay: >96.5% (HPLC)
• Quality Segment: 100
• form: solid
• mp: 165-168 °C (lit.)
• λmax: 323 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: COc1cc(NC(=O)c2ccccc2)c(OC)cc1N
• InChI: 1S/C15H16N2O3/c1-19-13-9-12(14(20-2)8-11(13)16)17-15(18)10-6-4-3-5-7-10/h3-9H,16H2,1-2H3,(H,17,18)
• InChI key: DDRCIGNRLHTTIW-UHFFFAOYSA-N

Beschreibung

Application

Fast Blue RR has been used to detect alkaline phosphatase activity.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appear black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable