Skip to Content
MilliporeSigma
  • Molecular epidemiology of Neisseria gonorrhoeae isolates from Saskatchewan, Canada: utility of NG-MAST in predicting antimicrobial susceptibility regionally.

Molecular epidemiology of Neisseria gonorrhoeae isolates from Saskatchewan, Canada: utility of NG-MAST in predicting antimicrobial susceptibility regionally.

Sexually transmitted infections (2014-02-08)
Sidharath D Thakur, Paul N Levett, Gregory B Horsman, Jo-Anne R Dillon
ABSTRACT

To investigate the molecular epidemiology of isolates of Neisseria gonorrhoeae from Saskatchewan, Canada, using Neisseria gonorrhoeae multi antigen sequence typing (NG-MAST), and to assess associations between antimicrobial susceptibility (AMS) and specific strain types (STs). 320 consecutive gonococcal isolates, collected between 2003 and 2008, were typed by NG-MAST. STs were grouped if one of their alleles was common and the other differed by ≤1% in DNA sequence. AMS was determined by agar dilution (CLSI) to seven antibiotics. N gonorrhoeae isolates were resolved into 82 individual NG-MAST STs and 18 NG-MAST ST groups with groups 25, 3655, 921, 3654, 3657 and 3656 comprising 53.4% (171/320) of the isolates. N gonorrhoeae isolates susceptible to all the tested antimicrobials were significantly (p<0.05) associated with ST 25 (87%). Other significant associations between ST and AMS included: ST 3654 and isolates with minimum inhibitory concentrations of ≥0.03 mg/L to third generation cephalosporins; ST 3711 (100%) and TRNG; and ST/group 3654 (43%) and chromosomal resistance to penicillin and tetracycline. Several NG-MAST STs/groups were significantly associated with isolates with chromosomal resistance to tetracycline. Isolates resistant to ciprofloxacin (n=5) and azithromycin (n=2) appeared as individual STs. Significant associations were observed among individual STs, sex and age of the patient, and regional and temporal distributions. Associations between N gonorrhoeae AMS and NG-MAST STs were identified and may be useful in predicting AMS regionally. Because STs in different countries vary considerably, the use of NG-MAST for the prediction of AMS globally requires further study.

MATERIALS
Product Number
Brand
Product Description

USP
Spectinomycin hydrochloride, United States Pharmacopeia (USP) Reference Standard
Azithromycin for peak identification, European Pharmacopoeia (EP) Reference Standard
Supelco
Ciprofloxacin HCl, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Azithromycin, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Azithromycin Identity, United States Pharmacopeia (USP) Reference Standard
Azithromycin, European Pharmacopoeia (EP) Reference Standard
Azithromycin for system suitability, European Pharmacopoeia (EP) Reference Standard
USP
Azithromycin, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Tetracycline, 98.0-102.0% (HPLC)
Sigma-Aldrich
Spectinomycin dihydrochloride pentahydrate, powder, γ-irradiated
Sigma-Aldrich
Spectinomycin dihydrochloride pentahydrate, potency: ≥603 μg per mg
Sigma-Aldrich
Spectinomycin dihydrochloride pentahydrate, powder, BioReagent, suitable for cell culture
Supelco
Spectinomycin dihydrochloride pentahydrate, VETRANAL®, analytical standard
Sigma-Aldrich
Tetracycline, 98.0-102.0% (HPLC)
USP
Ceftriaxone sodium, United States Pharmacopeia (USP) Reference Standard
Ceftriaxone sodium, European Pharmacopoeia (EP) Reference Standard
Supelco
Tetracycline hydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Tetracycline hydrochloride, VETRANAL®, analytical standard
Supelco
Ciprofloxacin, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Azithromycin dihydrate, ≥98% (HPLC)
Sigma-Aldrich
Azithromycin
Sigma-Aldrich
Ciprofloxacin, ≥98% (HPLC)
Sigma-Aldrich
Tetracycline hydrochloride, meets USP testing specifications
Sigma-Aldrich
Tetracycline hydrochloride, ≥95% (European Pharmacopoeia HPLC assay)
Sigma-Aldrich
Tetracycline hydrochloride, powder
Sigma-Aldrich
Ceftriaxone disodium salt hemi(heptahydrate), third-generation cephalosporin antibiotic
Sigma-Aldrich
Tetracycline hydrochloride, powder, BioReagent, suitable for cell culture
Tetracycline hydrochloride, European Pharmacopoeia (EP) Reference Standard
Ciprofloxacin, European Pharmacopoeia (EP) Reference Standard
Ciprofloxacin hydrochloride for peak identification, European Pharmacopoeia (EP) Reference Standard