Skip to Content
MilliporeSigma
  • Dopamine receptors of the rodent fastigial nucleus support skilled reaching for goal-directed action.

Dopamine receptors of the rodent fastigial nucleus support skilled reaching for goal-directed action.

Brain structure & function (2023-08-24)
Violeta-Maria Caragea, Marta Méndez-Couz, Denise Manahan-Vaughan
ABSTRACT

The dopaminergic (DA) system regulates both motor function, and learning and memory. The cerebellum supports motor control and the acquisition of procedural memories, including goal-directed behavior, and is subjected to DA control. Its fastigial nucleus (FN) controls and interprets body motion through space. The expression of dopamine receptors has been reported in the deep cerebellar nuclei of mice. However, the presence of dopamine D1-like (D1R) and D2-like (D2R) receptors in the rat FN has not yet been verified. In this study, we first confirmed that DA receptors are expressed in the FN of adult rats and then targeted these receptors to explore to what extent the FN modulates goal-directed behavior. Immunohistochemical assessment revealed expression of both D1R and D2R receptors in the FN, whereby the medial lateral FN exhibited higher receptor expression compared to the other FN subfields. Bilateral treatment of the FN with a D1R antagonist, prior to a goal-directed pellet-reaching task, significantly impaired task acquisition and decreased task engagement. D2R antagonism only reduced late performance post-acquisition. Once task acquisition had occurred, D1R antagonism had no effect on successful reaching, although it significantly decreased reaching speed, task engagement, and promoted errors. Motor coordination and ambulation were, however, unaffected as neither D1R nor D2R antagonism altered rotarod latencies or distance and velocity in an open field. Taken together, these results not only reveal a novel role for the FN in goal-directed skilled reaching, but also show that D1R expressed in FN regulate this process by modulating motivation for action.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Dopamine D2 Receptor Antibody, serum, Chemicon®
Sigma-Aldrich
Anti-Dopamine D1A Receptor Antibody, CT, cytoplasmic, Chemicon®, from rabbit