ZRB1589

Anti-Cdk2 Antibody, clone 1B21 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Cyclin-dependent kinase 2;EC:2.7.11.22;Cell division protein kinase 2;p33 protein kinase)
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B21, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 33.93 kDa; observed mol wt ~34 kDa
• purified by: using Protein A
• species reactivity: rat, human, mouse
• species reactivity (predicted by homology): monkey, canine, feline, bovine
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_001277159
• UniProt accession no.: P24941
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B21 is a Rabbit recombinant monoclonal antibody that specifically detects Cyclin-dependent kinase 2 (Cdk2). It targets an epitope within 25 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal half of human Cyclin-dependent kinase 2 (Cdk2).

Application

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Cdk2 in HEK293 cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Cdk2 in lysates from RAW264.7 cells, Rat kidney tissue, and MCF-7 cells.

Affinity Binding Assay: A representative lot of this antibody bound Cdk2 with a KD of 3.7 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cdk2 in HeLa and NIH 3T3 cells..

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cdk2 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Cyclin-dependent kinase 2 (UniProt: P24941;also known as EC:2.7.11.22, Cell division protein kinase 2, p33 protein kinase) is encoded by the CDK2 (also known as CDKN2) gene (Gene ID: 1017) in human. Cdk2 is a multifunctional serine/threonine-protein kinase that is involved in the control of the cell cycle and plays a role in germ cell development. It is localized in the nucleus, cytoplasm, and endosomes. It is considered to be important in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells. Its activity is reported to be maximum during S phase and G2 phase and it is activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition. It is subsequently activated by cyclin A2 during the late stages of DNA replication to drive the transition from S phase to G2. Cdk2 phosphorylation at threonine 160 by Cdk7 in a Cdk activating kinase (CAK) complex promotes its kinase activity. However, phosphorylation at threonine 14 and tyrosine 15 during S and G2 phases inactivates the enzyme and dephosphorylation by CDC25A is required for its activation. Active Cdk2 is reported to phosphorylate numerous substrates, including catenin b1, Ubiquitin Specific Peptidase 37 (USP37), p53/TP53, nucleophosmin 1, CDK7, RB1, MYC, and BRCA2. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Palmer, N., et al. (2020). PLoS Biol. 18(10): e3000903).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable