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About This Item
Pricing and availability is not currently available.
Quality Segment
form
powder
technique(s)
molecular cloning: suitable, transformation: suitable
application(s)
microbiology
storage temp.
room temp
Application
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants.
Preparation Note
Dissolve 1.46 g of supplement per liter of medium.
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
1 of 1
This Item | |||
|---|---|---|---|
| description without histidine, leucine, and tryptophan | description without uracil, leucine, and tryptophan | description without uracil | description without histidine, leucine, tryptophan, and adenine |
| form powder | form powder | form powder | form powder |
| technique(s) transformation: suitable | technique(s) transformation: suitable | technique(s) - | technique(s) transformation: suitable |
| application(s) microbiology | application(s) microbiology | application(s) food and beverages | application(s) microbiology |
| storage temp. room temp | storage temp. room temp | storage temp. - | storage temp. room temp |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Dam. 1
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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