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T5574
Terrific Broth
Liquid microbial growth medium
Synonym(s):
TB medium, Tartoff – Hobbs medium
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About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
Pricing and availability is not currently available.
grade
Molecular Biology
Quality Segment
description
Liquid Medium
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.
Application
Terrific Broth has been used for non-selective cultivation of E. coli strains for cloning, high-yield DNA plasmid production, and high-yield production of recombinant proteins. citation It is also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
Terrific Broth liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Other Notes
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L Glycerol
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L Glycerol
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This Item | |||
|---|---|---|---|
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) - |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| form liquid | form powder | form liquid | form powder |
| sterility sterile; 0.2 μm filtered | sterility non-sterile | sterility sterile; 0.2 μm filtered | sterility non-sterile |
| application(s) microbiology | application(s) agriculture | application(s) microbiology | application(s) food and beverages |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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What are the alternatives for T5574?
What are the alternatives for T5574?
1 answer-
Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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