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Key Documents

SRP4988

Sigma-Aldrich

PEDF human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym(s):

EPC-1, EPC1, PIG35, Pigment epithelium-derived factor, Serpin-F1, SerpinF1

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About This Item

MDL number:
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

~44.5 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | hybridoma: suitable
western blot: suitable

impurities

endotoxin, tested

suitability

suitable for Western blot

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PEDF(5176)

General description

Pigment epithelium-derived factor (PEDF) is a non-inhibitory serpin. It is a 50,000Da glycoprotein created and secreted in many tissues all the way through the body. The gene encoding this protein is localized on human chromosome 17p13.3. Recombinant human PEDF produced in Escherichia coli is a single, non-glycosylated, polypeptide chain containing 400 amino acids and having a molecular mass of 44.5kDa.
Research Area: Apoptosis
Pigment epithelium-derived factor (PEDF) is a multifunctional glycoprotein that belongs to the serine proteinase inhibitor (serpin) family. This protein is widely expressed across various tissues and organs in mammals and is characterized by a reactive center loop (RCL), a common structural feature of serpins. PEDF plays a crucial role in maintaining numerous physiological functions. SERPINF1 mRNA, which encodes PEDF, exhibits high expression levels in both human fetal and adult livers, as well as in adult human bone marrow.

Application

Pigment epithelium-derived factor (PEDF) has been used in cell culture for the production of pericytes and in Western blotting.

Biochem/physiol Actions

Pigment epithelium-derived factor (PEDF) has neurotrophic, anti-angiogenic and anti-tumorigenic properties. A key component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. Additionally, it is capable of inhibiting the activity of angiogenic factors such as vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2). The recognition of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. The unique range of PEDF activities associate it as a potential therapeutic agent for the treatment of vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF in addition has the potential to be functional in the treatment of various angiogenesis-related diseases including a number of cancers.
Pigment epithelium-derived factor (PEDF) protein is vital for various physiological and pathophysiological processes, including fibrogenesis and inflammation. A deficiency in PEDF is recognized as contributing to the development of several disease processes.

Physical form

The sterile filtered, concentrated (1 mg/ml) protein solution was lyophilized from 20 mM PBS and 150 mM NaCl, pH-7.4.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in sterile dH2O to a concentration of 0.1 -1 mg/mL and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pigment Epithelium-Derived Factor (PEDF) hypersensitivity in CAR & AMD
Charles Thirkill
Investigative Ophthalmology & Visual Science, 54 (2013)
Pigment epithelium-derived factor downregulates vascular endothelial growth factor (VEGF) expression and inhibits VEGF-VEGF receptor 2 binding in diabetic retinopathy.
Zhang SX
Journal of Molecular Endocrinology, 37(1), 1-12 (2006)
Pigment epithelium-derived factor: clinical significance in estrogen-dependent tissues and its potential in cancer therapy.
Franco-Chuaire ML
Iranian Journal of Basic Medical Sciences, 18(9), 837-855 (2015)
Pigment epithelium-derived factor behaves like a noninhibitory serpin. Neurotrophic activity does not require the serpin reactive loop.
Becerra SP
The Journal of Biological Chemistry, 270(43), 25992-25999 (1995)
PEDF and its roles in physiological and pathological conditions: implication in diabetic and hypoxia-induced angiogenic diseases
He X, et al.
Clinical Science, 128(11), 805-823 (2015)

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