SRP4166

IL-13 from rat

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(s): ALRH, BHR1, IL-13, NC300, P600, interleukin 13
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00675503

Properties

• biological source: rat
• recombinant: expressed in E. coli
• assay: ≥95% (HPLC), ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: ~12.7 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: P42203
• UniProt accession no.: P42203
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: rat ... Il13(116553)

Description

General description

IL13 (interleukin 13) is a classic cytokine produced by Th2 (T-helper) cells. It was originally identified as an inflammatory cytokine production-inhibiting T-cell derived cytokine. Recombinant rat IL-13 is an 11.9 kDa protein containing 109 amino acid residues.

Biochem/physiol Actions

IL13 (interleukin 13) is up-regulated during parasitic infections, and participates in host′s protective immunity against Schistosoma japonicum. This protein promotes eosinophil activation resulting in elevated specific IgE against adult and larval antigens. During acute infection it promotes egg expulsion through increased intestinal contractility. It acts as a liver protective agent where it promotes formation of liver granulomas in a CD4⁺ T cell-mediated manner, which results in liver fibrosis. In airway disorders, such as asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis lung disease, IL13 promotes the formation of airway goblet cells, resulting in excess mucus secretion, which in turn, is regulated by IL13-activated autophagy.

Physical form

Sterile filtered and lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can be diluted into other aqueous buffers.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable