SRP0193

PARP2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

• Synonym(s): ADPRT2, ADPRTL3, NAD(+) ADP-ribosyltransferase 2, Poly (ADP-ribose) Polymerase 2
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥60% (SDS-PAGE)
• form: aqueous solution
• mol wt: 92 kDa
• packaging: pkg of 10 μg
• manufacturer/tradename: Sigma-Aldrich
• concentration: >0.02 mg/mL
• technique(s): inhibition assay: suitable
• solubility: water: soluble
• NCBI accession no.: NM_005484
• UniProt accession no.: Q9UGN5
• application(s): life science and biopharma
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PARP2(10038)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable