SAB4600399
Anti-Rabbit IgG (H+L), F(ab′)2 fragment, CF™640R antibody produced in goat
~2 mg/mL, affinity isolated antibody, buffered aqueous solution
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
CF™ 640R conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rabbit
concentration
~2 mg/mL
technique(s)
flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1-10 μg/mL
western blot: suitable
fluorescence
λex 642 nm; λem 662 nm
storage temp.
−20°C
target post-translational modification
unmodified
General description
This product is prepared by labeling high quality F(ab′)2 fragment of goat anti-rabbit IgG (H+L) with the far-red fluorescent dye CF640R. CF640R is a novel rhodamine-based dye spectrally very similar to Cy5 and Alexa Fluor 647. CF640R is much brighter than Cy5 and at least as bright as Alexa Fluor 647 when excited at 633 or 635 nm. However, the most important advantage of CF640R is its exceptional photostability. CF640R is far more photostable than the cyanine-based dyes Cy5 and Alexa Fluor 647. It is also more photostable than Atto 647N, a dye that has been extensively used in single molecule-based imaging. CF640R is also extremely water-soluble and pH-insensitive. These properties make CF640R a much better alternative to Cy5, Alexa Fluor 647 and other spectrally similar dyes.
Specificity
Binds with whole molecule rabbit IgG and the light chains of other rabbit immunoglobulins.
Immunogen
rabbit IgG(H+L)
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.
Preparation Note
Protect from light. The antibody solution should be gently mixed before use.
Legal Information
This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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