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Sphingomyelinase from Staphylococcus aureus

Name: Sphingomyelinase from <I>Staphylococcus aureus</I>
Brand: SIGMA

buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)

Synonym(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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About This Item

CAS Number:

9031-54-3

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.1.4.12 (BRENDA, IUBMB)

MDL number:

MFCD00132362

Specific activity:

100-300 units/mg protein (Lowry)

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Properties

form

buffered aqueous glycerol solution

Quality Segment

200

specific activity

100-300 units/mg protein (Lowry)

storage temp.

2-8°C

Description

Application

Sphingomyelinase from Staphylococcus aureus has been used to:

induce neurotoxicity in rat cortical cultures to study the protective effects of minocycline
determine the concentration of sphingomyelin from serum samples
enhance sphingomyelinase activity to study PARK9-mediated exosome biogenesis

Biochem/physiol Actions

Bacterial sphingomyelinase is active at neutral pH. When used in cell culture in vitro, it hydrolyzes the sphingomyelin on the outer leaflet of the plasma membrane and produces ceramide that is lipid-soluble. Sphingomyelinase is the key enzyme in the sphingomyelinase/ceramide pathway, which is implicated in the pathogenesis of several neurodegenerative disorders.

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Physical form

Solution in 50% glycerol containing 0.25 M phosphate buffer, pH 7.5

Other Notes

One unit will hydrolyze 1.0 μmol of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

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This Item	567735	P8804	WH0027293M1
Sigma-Aldrich S8633 Sphingomyelinase from Staphylococcus aureus	Sigma-Aldrich 567735 Sphingosylphosphoryl-choline	Sigma-Aldrich P8804 Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus	Sigma-Aldrich WH0027293M1 Monoclonal Anti-SMPDL3B antibody produced in mouse
description buffered aqueous glycerol solution, 100-300 units/mg protein (Lowry)	description SPC is the deacylated derivative of sphingomyelin known to accumulate in neuropathic Niemann-Pick disease type A.	description buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)	description clone 5E12, purified immunoglobulin, buffered aqueous solution
specific activity 100-300 units/mg protein (Lowry)	specific activity -	specific activity ≥1,000 units/mg protein (Lowry)	specific activity -
form buffered aqueous glycerol solution	form solid	form buffered aqueous glycerol solution	form buffered aqueous solution
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 100

Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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