P8615

Phosphoinositide 3-kinase p110γ human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

• Synonym(s): PI3K p110γ human, Phosphatidylinositol 3-kinase p110γ human
• UNSPSC Code: 12352207
• NACRES: NA.32
• MDL number: MFCD03457993
• Specific activity: ~4.5 units/mg protein
• Assay: ≥95% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected insect cells

Properties

• recombinant: expressed in baculovirus infected insect cells
• Quality Segment: 200
• assay: ≥95% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: ~4.5 units/mg protein
• UniProt accession no.: P48736
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PIK3CG(5294)

Description

General description

Phosphatidylinositol 3-kinase p110γ /phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit γ (PIK3CG) gene, with 12 exons spanning 43 kb on genomic DNA, is mapped to human chromosome 7q22.3. PIK3CG belongs to the family of genes coding for enzymes that phosphorylate the 3′-hydroxyl of phosphatidylinositol.

Biochem/physiol Actions

PI 3-kinases have been implicated in diverse cellular responses triggered by mammalian cell surface receptors. Inhibits NF-κB, AP-1, and STAT1α mediated gene expression induced by IFN-γ resulting in inhibition of COX-2 and NOS ll (iNOS) expression.

PI 3-kinases have been implicated in diverse cellular responses triggered by mammalian cell surface receptors. Inhibits NF-κB, AP-1, and STAT1α mediated gene expression induced by IFN-γ resulting in inhibition of COX-2 and NOS ll (iNOS) expression.

Phosphatidylinositol 3-kinase p110γ is implicated in the development of eosinophilic inflammation. Thus, inhibition of PI3Kγ expression can be considered as a potential therapeutic method for treating eosinophil-related diseases, including asthma. Knockdown of PIK3CG gene inhibits the PI3K-Akt/PKB signaling pathway, which is essential for tumorigenesis and the progression of colorectal cancers. Mutation in the gene is associated with the development of autistic disorder.

Physical form

Solution in 10 mM HEPES, pH 7.5, 100 mM NaCl, 0.5 mM MgCl2, 50% glycerol.

Other Notes

1 unit will transfer 1 nmol of phosphate/min, using phosphatidylinositol as substrate.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable