P4630

Protease from bovine pancreas

Type I, ≥5 units/mg solid

• CAS Number: 9001-92-7
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-642-4
• NACRES: NA.54
• MDL number: MFCD00132092
• Specific activity: ≥5 units/mg solid
• Biological source: bovine pancreas

Properties

• biological source: bovine pancreas
• Quality Segment: 200
• type: Type I
• form: powder
• specific activity: ≥5 units/mg solid
• storage temp.: −20°C

Description

Application

Non-specific Protease Activity Assay Video

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Product P4630 is from bovine pancreas. Protease from bovine pancrease (type I) has been used for the extraction of hemicellulose.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Analysis Note

Crude

Other Notes

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves