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Phosphatase, Alkaline from Escherichia coli

Name: Phosphatase, Alkaline from <I>Escherichia coli</I>
Brand: SIGMA

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

Synonym(s):

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS Number:

9001-78-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-631-4

MDL number:

MFCD00131849

EC Number:

3.1.3.1 (BRENDA, IUBMB)

eCl@ss:

42010105

Specific activity:

20-50 units/mg protein (in glycine buffer)

Biological source:

Escherichia coli

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Properties

biological source

Escherichia coli

Quality Segment

200

form

buffered aqueous glycerol solution

specific activity

20-50 units/mg protein (in glycine buffer)

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

Description

Application

Phosphatase, Alkaline from Escherichia coli has been used:

in fluorometric assay to determine its enzyme activity
in the dephosphorylating enzyme cocktail for dephosphorylation of peptides
in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.

Biochem/physiol Actions

Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Physical form

Solution in 5 mM Tris-HCl, pH 7.4, 0.5 mM MgCl₂, 0.5 mM ZnCl₂, 50% (w/v) glycerol

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Disclaimer

Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75°C for 10 minutes.

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This Item	P4252	P5931	A2356
Sigma-Aldrich P4069 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich P4252 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich P5931 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich A2356 Phosphatase, Alkaline from bovine intestinal mucosa
description buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)	description ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)	description lyophilized powder, 30-60 units/mg protein (in glycine buffer)	description ≥5,500 DEA units/mg protein
biological source Escherichia coli	biological source Escherichia coli	biological source -	biological source -
specific activity 20-50 units/mg protein (in glycine buffer)	specific activity 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)	specific activity 30-60 units/mg protein (in glycine buffer)	specific activity ≥5,500 DEA units/mg protein
form buffered aqueous glycerol solution	form ammonium sulfate suspension	form lyophilized powder	form (Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200
foreign activity DNase, RNase, none detected	foreign activity -	foreign activity -	foreign activity -

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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