P1189

Polymyxin B

liquid, suitable for cell culture, BioReagent

• Synonym(s): Aerosporin, PMB, PMX, Polymyxin, Polymyxin B
• UNSPSC Code: 12352207
• NACRES: NA.76

Properties

• Product Name: Polymyxin B ready made solution, Cell culture tested, 17 mg/mL in H₂O
• Quality Segment: 200
• form: liquid
• concentration: 17 mg/mL in H₂O
• storage temp.: 2-8°C

Description

General description

Polymyxin B (PMB) is a cyclic cationic lipopeptide antibiotic originally produced by soil bacterium Bacillus polymyxa. Polymyxin B binds specifically to gram negative bacteria by interacting with the anionic lipopolysaccharide (LPS, or endotoxin). Polymyxin B interferes with the outer membrane normal function causing leakage and enhanced uptake of the antibiotic.

Polymyxin B has several applications:

• It is used to neutralize LPS contamination in vitro and in vivo. Its neutralizing effect is dose-dependent. In vitro experiments showed that PMB inhibits a septic shock by endotoxin inactivation.
• Cell cultures can be treated with PMB (100U/mL) to avoid early contaminations. PMB is considered a broad spectrum antibiotic and is used to treat infections caused by bacteria resistant to a combination of Penicillin and Streptomycin.
• PMB is also used as negative and positive control in minimum microbicidal concentration (MIC) assay.

Working concentration:
The suggested concentration of PMB in cell culture mediums is 100U/mL. Therefore, it is recommended to dilute PMB ready made solution 1:1000.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2