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Phenolphthalein β-D-glucuronide

Name: Phenolphthalein β-<SC>D</SC>-glucuronide
Brand: SIGMA

β-glucuronidase substrate

Synonym(s):

Phenolphthalein β-D-glucosiduronic acid, Phenolphthalein glucuronic acid, Phenolphthalein mono-β-D-glucosiduronic acid

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About This Item

Empirical Formula (Hill Notation):

C₂₆H₂₂O₁₀

CAS Number:

15265-26-6

Molecular Weight:

494.45

UNSPSC Code:

12352204

NACRES:

NA.32

PubChem Substance ID:

24898108

MDL number:

MFCD00135881

Beilstein/REAXYS Number:

68526

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Properties

Quality Segment

200

assay

≥97% (TLC)

form

powder

solubility

H₂O: soluble 100 mg/mL

storage temp.

−20°C

SMILES string

O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)C3(OC(=O)c4ccccc34)c5ccc(O)cc5

InChI

1S/C26H22O10/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32/h1-12,19-22,25,27-30H,(H,31,32)/t19-,20-,21+,22-,25+,26?/m0/s1

InChI key

FXJYOZKDDSONLX-XADSOVDISA-N

Description

Application

β-glucuronidase activity was determined by the amount of phenolphthalein released from 0.01 M henolphthalein β-D-glucuronide.

Phenolphthalein β-D-glucuronide has been used as a substrate for the detection of β-D-glucuronidase activity in fecal bacteria.

Biochem/physiol Actions

Phenolphthalein β-D-glucuronide is a chromogenic substrate for β-glucuronidase enzyme and the resultant colored product of the reaction is measured at 552 nm.

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1 of 1

This Item	P0376	78555	N1627
Sigma-Aldrich P0501 Phenolphthalein β-D-glucuronide	Sigma-Aldrich P0376 Phenolphthalein β-D-glucuronide sodium salt	Sigma-Aldrich 78555 Phenyl-β-D-glucuronide	Sigma-Aldrich N1627 4-Nitrophenyl β-D-glucuronide
assay ≥97% (TLC)	assay ≥90% (TLC)	assay ≥99.0% (HPLC)	assay ≥98% (TLC)
solubility H₂O: soluble 100 mg/mL	solubility H₂O: soluble 5 mg/mL	solubility -	solubility H₂O: soluble 100 mg/mL, clear
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 200
form powder	form powder	form powder	form powder
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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