Skip to Content
MilliporeSigma
All Photos(1)

Documents

MAK336

Sigma-Aldrich

D-Lactate Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

D-Lactate Detection Kit

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

input

cell medium
serum
plasma

detection method

colorimetric

relevant disease(s)

neurological disorders; gastrointestinal diseases

storage temp.

−20°C

General description

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

Application

The D-Lactate Assay Kit may be used for:
  • Gastrointestinal Disease Research
  • Neurological Disease Research

Features and Benefits

Enhanced Detection Range: Detect D-Lactate levels accurately across a wide range, from 0.05 mM to 2 mM, using only 20 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 20 minute room temperature reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

Suitable for D-lactate in serum, plasma, and cell media samples.

Principle

The D-Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

related product

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service