This product is not expected to present any major stability, solubility, aggregation, or conformational issues when reconstituted from pH 8.0 to approximately pH 7.4. This material is lyophilized from 0.02 M Tris, 0.13 M glycine, 0.08 M trehalose at pH 8.0 and is soluble to 10 mg/mL. Denaturation is noted only under acidic conditions below pH 4, not at near-physiological pH. This aligns with α2-macroglobulin being a native plasma protein and literature describing purified human α2M in near-physiological buffers around pH 7.2–7.4 while retaining native/functional properties. The main consideration is careful handling after reconstitution, as purified α2-macroglobulin can become heterogeneous or lose activity during freezing, lyophilization, prolonged storage, or repeated freeze–thaw cycles. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/182/570/m6159dat.pdf
M6159
α2-Macroglobulin from human plasma
BioUltra, ≥98% (SDS-PAGE)
Synonym(s):
α2-M
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About This Item
Pricing and availability is not currently available.
biological source
human plasma
Quality Segment
product line
BioUltra
assay
≥98% (SDS-PAGE)
form
lyophilized powder
mol wt
~720 kDa (four glycoprotein subunits)
composition
Protein, 15-30% biuret
technique(s)
inhibition assay: suitable
solubility
water: soluble 10 mg protein/mL, clear, colorless
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... A2M(2)
Application
α2-Macroglobulin was used to study impaired lipoprotein receptor-mediated peripheral binding of plasma amyloid-β which is an early biomarker for mild cognitive impairment preceding Alzheimer′s disease. It was also used in the control of the classical and the MBL pathway of complement activation.
Inhibits all classes of endoproteases by forming a complex with the protease. When the protease cleaves the macroglobulin "bait" sequence, the macroglobulin rearranges and traps the protease.
Biochem/physiol Actions
α2-Macroglobulin (α2M) is a multifunctional protein that is a broad spectrum protease inhibitor. α2M is a large homotetrameric glycoprotein (720 kDa) that is connected by disulfide-linked dimers which non-covalently interact to give the quaternary structure. α2M is found in normal plasma at a concentration of 220-230 mg/dl accounting for 3-5% of the total plasma protein.
α2-Macroglobulin is found abundantly in plasma and interstitial fluids. The protease-α2-M balance plays an important role in mediating inflammatory tissue destruction. Serum levels of α2-M and protease-α2-M complexes are increased in patients with sepsis, emphysema, periodontitis, rheumatoid arthritis, and other inflammatory diseases, and oxidant inactivation of α2-M may contribute to tissue destruction during inflammation.
Serum levels of α2-Macroglobulin (α2-M) and protease-α2-M complexes are increased in patients with sepsis, emphysema, periodontitis, rheumatoid arthritis and other inflammatory diseases. It is hypothesized that the oxidant inactivation of α2-M contributes to tissue destruction in inflammation.
Packaging
Package size based on protein content
Physical form
Lyophilized from 0.02 M Tris, 0.13 M glycine, pH 8.0, and 0.08 M trehalose
Analysis Note
Plasma from each donor has been tested and found negative for antibody to HIV-1/HIV-2, antibody to HCV and HbSAg.
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This Item | ||
|---|---|---|
| description BioUltra, ≥98% (SDS-PAGE) | description A multifunctional, secreted glycoprotein that acts as a broad range irreversible protease inhibitor that forms a "trap" around most proteases. | description - |
| assay ≥98% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay - |
| biological source human plasma | biological source - | biological source - |
| Gene Information human ... A2M(2) | Gene Information - | Gene Information human ... A2M(2) |
| Quality Level 200 | Quality Level 100 | Quality Level - |
| form lyophilized powder | form lyophilized | form - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Hi, For downstream biophysical characterization to conditions closer to physiological plasma/serum environment, do you anticipate any stability/solubility/aggregation, or conformational issues if α2-macroglobulin is reconstituted from pH 8.0 to ~pH 7.4?
1 answer-
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