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M0644

Sigma-Aldrich

Minimum Essential Medium Eagle

Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

Application

Minimum essential medium Eagle has been used to:

  • maintain natural killer (NK) cell lines
  • culture Leishmania donovani strains
  • culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles

Quantity

Formulated to contain 10.1 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

also commonly purchased with this product

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supplement

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hannah Finke et al.
Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie, 79(5), 328-336 (2018-07-18)
Miniscrews are an important choice for orthodontic anchorage. Yet reports on failures do exist, and attempts have been made to elucidate the causes. Clinical outcomes may be compromised not only by the mechanical implications of miniscrew design and the location
C C Lachaud et al.
Cell death & disease, 5, e1304-e1304 (2014-06-27)
Recent studies suggested that the post-natal mesothelium retain differentiative potential of the embryonic mesothelium, which generates fibroblasts and vascular smooth muscle cells (VSMCs), in developing coelomic organs via epithelial-to-mesenchymal transition (EMT). Whether adult mesothelial cells (MCs) are able to give
Kyoko Fujisawa et al.
PLoS neglected tropical diseases, 15(7), e0009552-e0009552 (2021-07-20)
Visceral leishmaniasis (VL) is a zoonotic protozoal vector-borne disease that is a major public health challenge. In Argentina, canine (CVL) and human visceral leishmaniasis (HVL) have recently emerged. There is a lack of standardised diagnostic tests for CVL, which hinders
Giorgia Montalbano et al.
Nanomaterials (Basel, Switzerland), 10(9) (2020-09-02)
Bone-tissue regeneration induced by biomimetic bioactive materials is the most promising approach alternative to the clinical ones used to treat bone loss caused by trauma or diseases such as osteoporosis. The goal is to design nanostructured bioactive constructs able to
J A C Guedes et al.
Bone, 115, 68-82 (2017-12-01)
The discovery of osteocalcin, a protein synthetized by osteoblasts, as a hormone that has positive effects on insulin resistance, contributed to support the concept of bone as an endocrine organ. However, very little is known about the molecular pathways involved
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Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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