M0643

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): EMEM, MEM
• NACRES: NA.75
• UNSPSC Code: 12352207

Properties

• Quality Segment: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: HEPES: no; Earle’s salts (5% CO₂): yes; NaHCO₃: no; phenol red: yes; L-glutamine: yes; sodium pyruvate: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids. It is widely used as a media formulation for many cell culture studies. It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63, dispersed ventricular cells, and endometrial adenocarcinoma Ishikawa cells.

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable