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L2654

Lipopolysaccharides from Escherichia coli O26:B6

γ-irradiated, BioXtra, suitable for cell culture

Synonym(s):

LPS

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About This Item

CAS Number:
UNSPSC Code:
12352211
EC Number:
297-473-0
NACRES:
NA.75
MDL number:
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biological source

Escherichia coli (O26:B6)

Quality Segment

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

purified by

gel-filtration chromatography

technique(s)

cell culture | mammalian: suitable

impurities

<5% Protein (Lowry)

solubility

H2O: 5 mg/mL, slightly hazy

storage temp.

2-8°C

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Lipopolysaccharides from Escherichia coli 026:B6 was used to elicit the secretion of cytokines by human PBMC[1], murine bone marrow dendritic cells[2] and rat astrocytes.[3]

Biochem/physiol Actions

LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.

Preparation Note

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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This Item
L6529L4516L2762
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

biological source

Escherichia coli (O26:B6)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O127:B8)

biological source

Escherichia coli (O26:B6)

solubility

H2O: 5 mg/mL, slightly hazy

solubility

H2O: 5 mg/mL, faintly hazy to hazy

solubility

H2O: 5 mg/mL, slightly hazy

solubility

water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C


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pictograms

Skull and crossbones

signalword

Danger

hcodes

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. LPS(Product number:L 2654)はTLR2に結合しますか?

    1 answer
    1. Lipopolysaccarides from Escherichia coli O26:B6 are expected to only interact with Toll-like receptor 4 (TLR4). Similar lipopolysaccharides from Porphyromonas gingivalis have demonstrated the ability to bind with both TLR2 and TLR4. This observation is described more fully in the peer-reviewed publication, "Effects of serotype and species dependency of bacterial lipopolysaccharides in human melanocytes from lightly and darkly-pigmented skin," and in its associated citations. A link to this article from PubMed Central can be found here:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074968/

      Please see the link below to review product SMB00610, LPS from Porphyromonas gingivalis"
      https://www.sigmaaldrich.com/product/sigma/smb00610

      Helpful?

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