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5-(Iodoacetamido)fluorescein

Name: 5-(Iodoacetamido)fluorescein
Brand: SIGMA

≥90% purity (HPLC), powder

Synonym(s):

5-IAF

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About This Item

Empirical Formula (Hill Notation):

C₂₂H₁₄INO₆

CAS Number:

63368-54-7

Molecular Weight:

515.25

UNSPSC Code:

12171500

PubChem Substance ID:

329815467

NACRES:

NA.47

Beilstein/REAXYS Number:

6543244

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Properties

Product Name

5-(Iodoacetamido)fluorescein, ≥90% (HPLC)

Quality Segment

200

assay

≥90% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow to orange

solubility

DMSO: 5 mg/mL

fluorescence

λ_ex 492 nm; λ_em 515 nm (Mercaptoethanol adduct), λ_ex 493 nm; λ_em 522 nm (pH 9.2)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(NC(=O)CI)ccc45)c1

InChI

1S/C22H14INO6/c23-10-20(27)24-11-1-4-15-14(7-11)21(28)30-22(15)16-5-2-12(25)8-18(16)29-19-9-13(26)3-6-17(19)22/h1-9,25-26H,10H2,(H,24,27)

InChI key

UATCLPJEZJKNHE-UHFFFAOYSA-N

Description

General description

5-(Iodoacetamido)fluorescein is a thiol reactive fluorescein derivative.

Application

5-(Iodoacetamido)fluorescein is used for the synthesis of fluorescently labeled organelles (nuclei and nuclear matrices), proteins and peptides that include enzymes.

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This Item	D0531	F3651	201626
Sigma-Aldrich I9271 5-(Iodoacetamido)fluorescein	Sigma-Aldrich D0531 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride	Sigma-Aldrich F3651 Fluorescein 5(6)-isothiocyanate	Sigma-Aldrich 201626 Fluoresceinamine, isomer I
description ≥90% (HPLC)	description ≥90% (HPLC)	description ≥90% (HPLC)	description -
technique(s) titration: suitable	technique(s) -	technique(s) titration: suitable	technique(s) -
fluorescence λ_ex 492 nm; λ_em 515 nm (Mercaptoethanol adduct), λ_ex 493 nm; λ_em 522 nm (pH 9.2)	fluorescence λ_ex 495 nm; λ_em 516 nm	fluorescence -	fluorescence -
color yellow to orange	color yellow to orange	color yellow to orange	color -
solubility DMSO: 5 mg/mL	solubility -	solubility acetone: 1 mg/mL, clear, light yellow to yellow	solubility methanol: 5 mg/mL
form powder	form powder	form powder	form powder
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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