I3019

Interleukin-10 from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture

• Synonym(s): IL-10
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00212207

Properties

• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.01-1.50 ng/mL EC₅₀
• mol wt: ~18 kDa
• packaging: pkg of 5 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P18893
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: mouse ... Il10(16153)

Description

Application

Interleukin-10 from mouse has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.

Biochem/physiol Actions

Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1⁺ (CD5⁺⁾, and normal B cells. In human, the cellular sources of IL-10 consist of CD4⁺ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.

Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.

Regulates lymphoid and myeloid cell functions; blocks the activation of cytokine synthesis and several accessory functions of macrophages; enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein

Analysis Note

The biological activity of IL-10 is determined in a cell proliferation assay using MC/9 cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)