This product is formulated with hydrocortisone as the raw material, so it is not in the form of hydrocortisone hemisuccinate or hydrocortisone sodium succinate.
H0396
Hydrocortisone-Water Soluble
BioReagent, suitable for cell culture
Hydrocortisone-Water Soluble
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Synonym(s):
Cyclodextrin-encapsulated hydrocortisone
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About This Item
Biological source:
plant (wild yam)
Form:
crystalline
Pricing and availability is not currently available.
biological source
plant (wild yam)
Quality Segment
product line
BioReagent
form
crystalline
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 400 mg/mL
shipped in
ambient
storage temp.
room temp
SMILES string
C[C@]12CCC(=O)C=C1CC[C@H]3[C@@H]4CC[C@](O)(C(=O)CO)[C@@]4(C)C[C@H](O)[C@H]23
InChI
1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
InChI key
JYGXADMDTFJGBT-VWUMJDOOSA-N
Gene Information
human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)
General description
Hydrocortisone has the potential to induce the formation of blood-brain barrier properties in serum-free culture media. It is recommended for use in epithelial and endothelial adherent cell culture applications.
Application
Hydrocortisone-Water Soluble has been used as a supplement in Dulbecco′s modified Eagle′s medium (DMEM)/high glucose medium to culture mammary alveolar (MAC-T) cells. It has also been used to study its effect on cation absorption by semicircular canal duct epithelium via epithelial sodium channel.
Packaging
Package size based on hydrocortisone
1 of 1
This Item | |||
|---|---|---|---|
| biological source plant (wild yam) | biological source soybean | biological source synthetic (organic) | biological source non-animal source |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 500 |
| product line BioReagent | product line BioReagent | product line BioXtra | product line BioXtra |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form crystalline | form powder | form powder | form liquid |
| solubility H2O: 400 mg/mL | solubility chloroform/ethanol (1:1): 50 mg/mL, clear, colorless to very faintly yellow | solubility ethanol: 1 mg/mL, clear | solubility - |
signalword
Danger
hcodes
Hazard Classifications
Repr. 1A
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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What is the chemical form of hydrocortisone in this product? Is this hydrocortisone hemisuccinate OR Hydrocortisone sodium succinate?
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Dopo avre sciolto in acqua Hydrocortisone (H0396) a che temperatura deve essere conservato?
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Stock solutions may be refrigerated for up to 2 weeks. For long term storage, the stock solution may be stored at -20°C in aliquots for 6 - 12 months. Avoid repeated freeze/thaw cycles. This product is not sterile. Stock solutions should be filter sterilized using a 0.22 micron filter.
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Which temperature would you suggest to store "400 mg/mL stock solution"? and for how long? Do you suggest to prepare H0396 in a sterile environment and further filter working solution using the 0.22um filter before cell culture use?
1 answer-
Stock solutions may be refrigerated for up to 2 weeks. For long term storage we recommend -20 deg C in aliquots for 6 - 12 months. Avoid repleated freeze/thaw cycles. This product is not sterile. Stock solutions should be filter sterilized using a 0.22 micron filter.
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How do I calculate the molarity of product H0396, Hydrocortisone-Water Soluble?
1 answer-
Product H0396 (Hydrocortisone-Water Soluble) is a complex of Hydrocortisone and cyclodextrin. Cyclodextrin is used to aid in the solubility of Hydrocortisone into water. In order to prepare a molar solution of Hydrocortisone you will have to base it on the Hydrocortisone content in the solution and not the entire complex. Product H0396 is formulated to contain approximately 85 mg Hydrocortisone per gram of powder. Based on this average, if you are preparing a 400 mg/ml stock solution, there will be approximately 34 mg of Hydrocortisone in 1 ml. Below is the calculated molarity of Hydrocortisone when the complex is dissolved at 400 mg/ml in water. molecular weight X unknown molarity X volume = complex weight X complex ratio362.46 g/mol X Molarity X 1 ml = 400 mg X 0.085 Molarity = 0.094 MHydrocortisone molecular weight = 362.46 g/molmolarity (unknown) = mol/Lvolume = 1mlcomplex weight = 400 mgcomplex ratio = 85 mg Hydrocortisone/1 gm complex
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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