G5080

Sephadex^® G-50

Fine

• Synonym(s): Gel filtration resin, Sephadex G-50 Fine Medium
• CAS Number: 9048-71-9
• MDL number: MFCD00132771
• NACRES: NA.56
• UNSPSC Code: 23151817

Properties

• technique(s): affinity chromatography: suitable
• matrix active group: phase
• swelling: 1 g swells to 9-11 mL
• bead size: 20-80 μm
• application(s): life science and biopharma
• compatibility: Cytiva

Description

General description

Sephadex G-50 Fine is a widely used gel filtration resin designed for desalting and buffer exchange of biomolecules with a molecular weight greater than 30,000. Its small bead size contributes to higher efficiency in these processes. Different types of Sephadex vary in terms of cross-linking, resulting in differences in swelling and molecular fractionation range. Sephadex G-50 is one of five G-types available, ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 comes in four different particle sizes, such as Coarse, Medium, Fine, and Superfine.

Sephadex^® G-50 is a gel filtration medium used in affinity chromatography, protein chromatography, and gel filtration chromatography. Sephadex is a beaded gel prepared by crosslinking dextran with epichlorohydrin.
Fractionation Range (MW)
Globular Proteins: 1,500 - 30,000
Dextrans: 500 - 10,000

Application

Sephadex^® G-50 is suitable for use in:

• the separation of low and high molecular weight molecules
• affinity chromatography, protein chromatography, and gel filtration chromatography

Sephadex^® G-50 has been used:

• to remove the non-entrapped carboxyfluorescein (CF) from the liposome suspension
• in the purification of monoclonal antibody (mAb) humanized MN-14 by centrifuged size-exclusion chromatography
• in desalting the recombinant enzymes eluted in the fraction five and six

Features and Benefits

• Desalts, removes contaminants, and transfers to a new buffer in one step.
• Suitable for DNA purification from small molecules using gel filtration.
• Features a small bead size, resulting in shorter diffusion distances.
• Considered a classic gel filtration resin.

• Desalting with Sephadex is considered superior to dialysis because it saves time, has a low dilution factor, and recovers high activity even with minute amounts of sample

Other Notes

G5080-100G′s updated product number is GE17-0042-01
G5080-500G′s updated product number is GE17-0042-02

Legal Information

Sephadex is a registered trademark of Cytiva

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)