EMS0004

SOLu-Trypsin Protease

Ready-to-use stable solution for automation, suitable for mass spectrometry, recombinant, expressed in Pichia pastoris

• Synonym(s): Protein Digestion for Mass Spectrometry, Ready to Use Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Trypsin for Protein Digestion, rTrypsin
• UNSPSC Code: 12352204
• NACRES: NA.78
• Form: ready-to-use solution
• Recombinant: expressed in Pichia pastoris

Properties

• Product Name: SOLu-Trypsin, recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid
• recombinant: expressed in Pichia pastoris
• Quality Segment: 200
• grade: Proteomics Grade
• description: Activity:≥ 10,000 unit/mg protein
• form: ready-to-use solution
• suitability: suitable for mass spectrometry
• UniProt accession no.: P00761
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Trypsin is a pancreatic serine endoprotease. SOLu-Trypsin is made from recombinant trypsin, porcine sequence. It does not possess chymotryptic activity.

Application

Poster: Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

SOLu-Trypsin:

• has been used to digest the gel pieces for mass spectrometry identification of active deubiquitnases (DUBs)[1]
• has been used in trypsin sensitivity assay
• may be used for the digestion of wine proteins

Biochem/physiol Actions

• SOLu-Trypsin (EMS0004) is our exclusive, Advanced Proteomics Grade enzyme that is solution-stable for mass spectrometry.
• Designed to be stable in solution when refrigerated, SOLu-Trypsin can be used immediately without preparation.
• Other forms of trypsin require thawing or reconstitution, and must be discarded if not used immediately.
• SOLu-Trypsin allows excess product to be saved for future use, thus eliminating unnecessary waste and cost.
• It is formulated with a high-purity recombinant trypsin, free of chymotryptic activity, to ensure high fidelity digestion.

Trypsin is used in proteomics research for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. It hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues.

Features and Benefits

• Ready to use - no preparation, such as reconstitution or thawing, is required
• Fits seamlessly into established workflow - no need to modify protocols
• Eliminates waste - remains stable in the refrigerator after use so there is no need to discard excess product
• Recombinant, porcine sequence - no chymotryptic activity
• Stable for short-term use at room temperature in an autosampler or on a liquid handling robot

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable