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D8037

Driselase Basidiomycetes sp.

BioReagent, suitable for plant cell culture

Synonym(s):

Driselase from Basidiomycetes sp.

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About This Item

CAS Number:
UNSPSC Code:
10171502
EC Number:
286-055-3
NACRES:
NA.72
MDL number:
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product line

BioReagent

Quality Segment

form

powder

composition

Protein, ≥10% biuret

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

−20°C

Application

Driselase Basidiomycetes sp. has been used:

  • in spheroplast preparation from Coccomyxa cells
  • in a CRISPR/Cas9-based mutagenesis protocol for Brachypodium distachyon and its allopolyploid relative, Brachypodium hybridum
  • for cell wall digestion to perform whole-mount immunolocalization of Lotus japonicus root tissue

Biochem/physiol Actions

Driselase is a natural mixture of enzyme activities (fungal carbohydrates) used to digest plant cell walls to facilitate the maceration of plant materials, protoplast formation, and extraction processes. Driselase releases cell wall carbohydrates. This formulation contains enzyme activities of cellulose, endo-1,3-β-glucanase, and xylanase.

Other Notes

Crude powder containing laminarinase, xylanase and cellulase.

Legal Information

Driselase is a trademark of ASKA Animal Health Co. Ltd.

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This Item
D5417C1794D5912
technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

form

powder

form

powder

form

powder

form

powder

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

composition

Protein, ≥10% biuret

composition

-

composition

-

composition

-


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

  1. Hi, do you know dissolvability and the optimum pH of this enzyme?

    1 answer
    1. As per our Enzymatic Assay of XYLANASE Activity in DRISELASE (EC 3.2.1.8):
      Driselase (Xylanase) Enzyme Solution
      (Immediately before use, prepare a solution containing 0.075 – 0.15 units/ml of Xylanase in
      cold deionized water.) The pH information can also be found in the link below:
      https://www.sigmaaldrich.cn/deepweb/assets/sigmaaldrich/product/documents/124/166/d9515enz.pdf

      Helpful?

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