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D4522

Sigma-Aldrich

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

Synonym(s):

Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52

grade

for molecular biology

Quality Level

type

Type XV

form

lyophilized powder

storage temp.

−20°C

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General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Quantity

One mg DNA is approx. 20 A260 units.

Preparation Note

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Brian D Holt et al.
Advanced healthcare materials, 5(23), 3056-3066 (2016-12-08)
Synthetic biomaterials are poised to transform medicine; however, current synthetic options have yet to ideally recapitulate the desirable properties of native tissue. Thus, the development of new synthetic biomaterials remains an active challenge. Due to its excellent properties, including electrical
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.
H V APOSHIAN et al.
The Journal of biological chemistry, 237, 519-525 (1962-02-01)
Benjamin D Dickson et al.
Molecules (Basel, Switzerland), 24(8) (2019-04-24)
Poly(ADP-ribose)polymerase (PARP) inhibitors (PARPi) have recently been approved for the treatment of breast and ovarian tumors with defects in homologous recombination repair (HRR). Although it has been demonstrated that PARPi also sensitize HRR competent tumors to cytotoxic chemotherapies or radiotherapy
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Tissue engineering, 11(9-10), 1498-1505 (2005-11-02)
The ultimate goal of this research is to develop an injectable cell-scaffold system capable of permitting adipogenesis to abrogate soft tissue deficiencies resulting from trauma, tumor resection, and congenital abnormalities. The present work compares the efficacy of photopolymerizable poly(ethylene glycol)
Claudia Gomes et al.
Life science alliance, 4(11) (2021-09-11)
High levels of autoimmune antibodies are observed in COVID-19 patients but their specific contribution to disease severity and clinical manifestations remains poorly understood. We performed a retrospective study of 115 COVID-19 hospitalized patients with different degrees of severity to analyze

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