CHOK1

CHOZN^® CHO K1 Host Cell Line

Suspension-adapted in CD media

• UNSPSC Code: 12352200

Properties

• description: CHOK1 cell line derived from ECACC CHO K1
• Quality Segment: 200
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine γ serialdehyde. They undergo morphological changes in response to cholera toxin.

CHO-K1 cells derived from ECACC CHO K1 and adapted to suspension and serum-free, chemically defined media. Cells are cGMP banked in chemically defined, animal component-free EX-CELL^® CD CHO Fusion medium.

Biochem/physiol Actions

Hamster Chinese ovary. The parental CHO-K1 cell line was originated by Puck in 1957.

Physical form

CHOZN CHO K1 cells are provided to customers in vials containing 1 mL at 10⁷ cells/mL. Cells are banked in EX-CELL CD Fusion medium containing 4mM L glutamine and 7% DMSO.

Legal Information

The CHOZN CHO K1 cell line is sold for research use only. For use of this cell line in a commercial process, a commercial license must be taken.

CHOZN is a registered trademark of Merck KGaA, Darmstadt, Germany

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable