LL/2(LLc1)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90020104, mouse lung, Not specified

• Synonym(s): LLC1 Cells, Lewis lung carcinoma line 1 Cells
• UNSPSC Code: 41106514
• Biological source: mouse lung
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Not specified

Properties

• biological source: mouse lung
• packaging: tube of 5 μg 90020104-DNA-5UG, pkg of vial of cells 90020104-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Tumourigenicity, Metastasis and chemotherapeutic agent studies

Biochem/physiol Actions

Derived from the lung of a C57BL mouse implanted with a primary Lewis Lung carcinoma. The cells can be used as a model for metastasis and for studying the effects of chemotherapeutic agents.

Mouse C57BL Lewis lung carcinoma

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using mechanical technique (aspiration); 5% CO₂; 37°C. Cells detach as aggregates and are therefore difficult to count.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].