WI 38VA13 Subline 2RA
85062512, human lung (fetal), Epithelial
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About This Item
UNSPSC Code:
41106514
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product name
WI 38VA13 Subline 2RA, 85062512
biological source
human lung (fetal)
growth mode
Adherent
karyotype
2n = 46, hyperdiploid, modal no. 73-78
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian foetal lung, SV40 transformed
Cell Line Description
An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.
Application
Transformation and virus studies: SV40
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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