Skip to Content
MilliporeSigma
All Photos(3)

Key Documents

C3595

Sigma-Aldrich

Anti-Connexin-32 (106-124) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CMTX, Anti-CMTX1, Anti-CX32

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

MDL number:
MFCD01633266
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 27 kDa

species reactivity

mouse, human, rat

technique(s)

immunohistochemistry (frozen sections): 1:400 using frozen rat liver sections
western blot: 1:400 using a rat liver membrane preparation

UniProt accession no.

P08034

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GJB1(2705)
mouse ... Gjb1(14618)
rat ... Gjb1(29584)

General description

Connexins (Cx) are a multi-gene family of highly related proteins with molecular weights ranging from 26 to 70 kDa. The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops, and a C-terminal cytoplasmic tail of varying length. The 27kD connexin protein (connexin 32, Cx32) is expressed in several tissues.

Immunogen

synthetic human connexin-32 peptide (amino acids 106-124).

Application

Anti-Connexin 32 (265-279) antibody produced in rabbit is suitable for immunohistochemistry (frozen sections) at a dilution of 1:400 using frozen rat liver tissue. It is also suitable for western blot at a dilution of 1:400 using a rat liver membrane preparation.
Anti-Connexin-32 (106-124) antibody produced in rabbit has been used in:
  • western blotting
  • immunofluorescence
  • immunohistology

Biochem/physiol Actions

Cx32 mutations is associated with X-linked Charcot-Marie-Tooth (CMTX) disease, which leads to myelin disruption and axonal degeneration.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Effects of substitution of Cx43 by Cx32 on myocardial energy metabolism, tolerance to ischaemia and preconditioning protection
Rodriguez-Sinovas A, et al.
The Journal of Physiology, 588(7), 1139-1151 (2010)
The expression of mesenchymal, neural and haematopoietic stem cell markers in adult hepatocytes proliferating in vitro
Koenig S, et al.
Journal of Hepatology, 44(6), 1115-1124 (2006)
Diego López et al.
Experimental physiology, 94(10), 1088-1097 (2009-07-21)
Previous studies demonstrated that intercellular communication through endothelial, smooth muscle or myoendothelial connexin channels contributes to the control of vascular tone. At least four connexin types are present in the arterial wall. The aim of the present work was to
Transient, recurrent, white matter lesions in X-linked Charcot-Marie-Tooth disease with novel connexin 32 mutation
Hanemann CO, et al.
Archives of Neurology, 60(4), 605-609 (2003)
Sarah Koenig et al.
Journal of hepatology, 44(6), 1115-1124 (2006-02-07)
Cultured adult hepatocytes may be stimulated into clonal expansion. We raise the question whether adult hepatocytes proliferating in vitro recapitulate the early process of hepatic development. A non-enzymatic method was used to isolate hepatocytes free of contamination with non-parenchymal cells.
View All Related Papers

Articles

Carcinogenesis and Epigenetics

Cancer research has revealed that the classical model of carcinogenesis, a three step process consisting of initiation, promotion, and progression, is not complete.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service