A9205

Bovine Serum Albumin solution

30% in saline, fatty acid free, aseptically filled

• Synonym(s): Albumin solution from bovine serum, BSA solution
• CAS Number: 9048-46-8
• UNSPSC Code: 12352202
• NACRES: NA.27
• MDL number: MFCD00130384
• eCl@ss: 32160409
• Biological source: bovine
• Assay: 28-32% protein basis (biuret)
• Form: solution
• Technique(s): ELISA: suitable, electrophoresis: suitable, western blot: suitable
• Impurities: ≤0.01% free fatty acid
• Concentration: 28-32% protein (biuret), 30% in saline

Properties

• biological source: bovine
• Quality Segment: 200
• sterility: aseptically filled
• assay: 28-32% protein basis (biuret)
• form: solution
• purified by: cold ethanol fractionation
• does not contain: stabilizer or preservative
• origin: USA origin
• concentration: 28-32% protein (biuret), 30% in saline
• free fatty acid content: ≤0.01%
• technique(s): ELISA: suitable, electrophoresis: suitable, western blot: suitable
• impurities: ≤0.01% free fatty acid
• UniProt accession no.: P02769
• foreign activity: BT virus, none detected, VSV virus, none detected
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

General description

Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.

Application

Bovine Serum Albumin solution has been used:

• as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells
• as a component of hepatocyte recovery medium
• as a component of fusion and activation buffers for in vitro reconstructed sheep embryos

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis. It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation. Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry. BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.

Preparation Note

Initial fractionation by cold alcohol precipitation

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)