A7437
Acetate Kinase from Escherichia coli
lyophilized powder, ≥150 units/mg protein (biuret)
Synonym(s):
ATP:Acetate phosphotransferase
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
MDL number:
Specific activity:
≥150 units/mg protein (biuret)
Pricing and availability is not currently available.
form
lyophilized powder
Quality Segment
specific activity
≥150 units/mg protein (biuret)
composition
Protein, 5.0-20.0%
UniProt accession no.
foreign activity
adenosine triphosphatase and NADH oxidase ≤0.01%
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... ackA(946775)
General description
Acetate kinase belongs to acetate and sugar kinase/Hsc70/actin (ASKHA) structural superfamily.[1] It exists as a dimer and has nucleotide binding site in the cleft between N and C-terminal domain.
Application
Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation [2].
Acetate Kinase from Escherichia coli has been used in assessing acetate kinase activity during the fermatation process of Gluconacetobacter xylinus and in kinetic modeling and steady state studies.
Biochem/physiol Actions
Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA [3]. Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine.
Involved in the metabolism of propanoate, pyruvate and taurine
Physical form
Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol
Other Notes
One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.6 at 25 °C.
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This Item | |||
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| Gene Information Escherichia coli K12 ... ackA(946775) | Gene Information - | Gene Information Escherichia coli K12 ... glnA(948370) | Gene Information - |
| specific activity ≥150 units/mg protein (biuret) | specific activity 40-100 units/mg protein | specific activity 400-2,000 units/mg protein | specific activity - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form ammonium sulfate suspension |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. | UniProt accession no. - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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