A5588

Anti-polyHistidine−Alkaline Phosphatase antibody, Mouse monoclonal

clone HIS-1, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-polyHistidine, Monoclonal Anti-polyHistidine−Alkaline Phosphatase antibody produced in mouse, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag
• NACRES: NA.56
• UNSPSC Code: 12352203
• Conjugate: alkaline phosphatase conjugate
• Clone: HIS-1, monoclonal
• Application: WB
• Citations: 22

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: HIS-1, monoclonal
• form: buffered aqueous glycerol solution
• technique(s): western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody recognizes native and denatured-reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Immunogen

recombinant polyHistidine tagged fusion protein.

Application

Antibody is suitable for ELISA, immunoblotting and dot blotting assays.

Biochem/physiol Actions

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)