A3981

Anti-β-Amyloid antibody, Mouse monoclonal

clone BAM-10, purified from hybridoma cell culture

• Synonym(s): Anti-A-BETA, Anti-Amyloid β Precursor Protein, Clone BAM91
• MDL number: MFCD00803867
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BAM-10, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• packaging: antibody small pack of 25 μL
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using formic-acid treated tissue sections of human Alzheimer disease brain tissue; indirect ELISA: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: P05067
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... APP(351) , APP(351)

Description

General description

β-amyloid gene is located on human chromosome 21q21.

The β-amyloid precursor protein (APP) is cleaved sequentially by the proteolytic enzymes β-and γ-secretase to produce β-amyloid (Aβ) peptides with the Aβ1-42 and the Aβ1-40 forms being the most prevalent. Secreted Aβ peptides are degraded either via a re-uptake mechanism followed by endosomal degradation, or by an extracellular insulin degrading enzyme. Extracellular accumulation of Aβ leads to the formation of aggregates, fibrils and eventually amyloid deposits called neuritic plaques, which is the hallmark of Alzheimer′s disease (AD).

The antibody reacts specifically with β-amyloid protein. The epitope recognized by the antibody resides within amino acids 1-12 of the β-amyloid protein. It specifically stains amyloid plaques within the cortex and amyloid deposits in blood vessels using formic acid-treated, formalin-fixed, paraffin-embedded, and Methacarn-fixed sections of human Alzheimer′s disease (AD) brain tissue.

Immunogen

Synthetic β-amyloid peptide, conjugated to KLH.

Application

Monoclonal Anti-β-Amyloid antibody has been used in immunoprecipitation analysis, microarray-based immunoassay and microarray.

Mouse monoclonal anti-ABETA was used to treat old WT PDAPP mice with amyloid accumulation and learning deficits in an attempt to improve learning and decrease accumulation, however no response was observed.

The antibody is useful in immunohistochemistry, immunoblotting, ELISA, and competitive ELISA. Also, this antibody has been used to neutralize Aβ assemblies in brains of transgenic mice expressing a mutant form of amyloid precursor protein, and for in vivo deep tissue imaging using near-IR optical spectrum.

Biochem/physiol Actions

β-amyloid gene acts as the substrate of insulin-degrading enzyme (IDE). It plays a major role in the pathogenesis of Alzheimer′s disease (AD) and type 2 diabetes mellitus (DM2).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves