A2050

Avidin–FITC from egg white

buffered aqueous solution

• Synonym(s): Avidin–Fluorescein isothiocyanate
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00130585
• Form: buffered aqueous solution

Properties

• conjugate: FITC conjugate
• Quality Segment: 200
• form: buffered aqueous solution
• extent of labeling: 3-5.5 mol fluorochrome per mol protein
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL, indirect immunofluorescence: 1:200
• UniProt accession no.: P02701
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: chicken ... AVD(396260)

Description

General description

Avidin is homotetrameric protein (68 kDa) that is obtained from egg whites.

Application

Avidin-FITC from egg white has been used in:

• flow cytometry
• double immunostaining
• double immunofluorescence
• immunnohistochemistry

To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.

Physical form

Solution in 0.01 M phosphate buffered saline containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide.

Preparation Note

Affinity purified protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)