Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

A1420

Sigma-Aldrich

Ames′ Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41161501
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: 1.081 g/L (Dextro)
NaHCO3: no
L-glutamine: 0.073 g/L

shipped in

ambient

storage temp.

2-8°C

General description

Ames′ Medium has been formulated to support retinal tissue in relatively short-term culture. It is suitable for maintaining central nervous system tissue in vitro.

Application

Ames′ Medium has been used to maintain rat/mice/macaque monkey eye for the isolation of retina.

Quantity

Formulated to contain 8.8 grams of powder per liter of medium.

Reconstitution

Supplement with 1.9 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Sabrina Asteriti et al.
Visual neuroscience, 34, E006-E006 (2017-10-03)
Rod-cone gap junctions mediate the so-called "secondary rod pathway", one of three routes that convey rod photoreceptor signals across the retina. Connexin 36 (Cx36) is expressed at these gap junctions, but an unidentified connexin protein also seems to be expressed.
Michael B Manookin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(16), 4136-4150 (2008-04-18)
Cone signals divide into parallel ON and OFF bipolar cell pathways, which respond to objects brighter or darker than the background and release glutamate onto the corresponding type of ganglion cell. It is assumed that ganglion cell excitatory responses are
John M Nagarah et al.
Frontiers in neuroscience, 9, 384-384 (2015-11-06)
Multielectrode arrays (MEAs) allow for acquisition of multisite electrophysiological activity with submillisecond temporal resolution from neural preparations. The signal to noise ratio from such arrays has recently been improved by substrate perforations that allow negative pressure to be applied to
B Ameline et al.
Gene therapy, 24(11), 735-741 (2017-09-08)
Several preclinical studies have investigated the potential of algal channelrhodopsin and human melanopsin as optogenetic tools for vision restoration. In the present study, we assessed the potentially deleterious effects of long-term expression of these optogenes on the diseased retina in
Antoine Chaffiol et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 25(11), 2546-2560 (2017-08-16)
The majority of inherited retinal degenerations converge on the phenotype of photoreceptor cell death. Second- and third-order neurons are spared in these diseases, making it possible to restore retinal light responses using optogenetics. Viral expression of channelrhodopsin in the third-order

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service