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Sigma-Aldrich

Atto 550 NHS ester

BioReagent, suitable for fluorescence

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About This Item

MDL number:
UNSPSC Code:
12352108
NACRES:
NA.25

product line

BioReagent

Quality Level

Assay

≥90.0% (HPLC)
≥90.0% (degree of coupling)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 553.0-559.0 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550 NHS ester is a reagent used to label molecules such as proteins with the Atto 550 fluorescence probe. This probe may be used in the design of fluoresence resonance transfer (FRET) detection systems.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Andriy Mokhir et al.
Inorganic chemistry, 44(16), 5661-5666 (2005-08-03)
A concept of fluorescent metal ion sensing with an easily tunable emission wavelength is presented and its principle demonstrated by detection of Cu(2+). A fluorescein dye was chemically modified with a metal chelating group and then attached to the terminus
Modeling colitis-associated cancer with azoxymethane (AOM) and dextran sulfate sodium (DSS).
Thaker AI, Shaker A, Rao MS, Ciorba MA.
Journal of Visualized Experiments (2012)
Thorben Cordes et al.
Physical chemistry chemical physics : PCCP, 13(14), 6699-6709 (2011-02-12)
Modern fluorescence microscopy applications go along with increasing demands for the employed fluorescent dyes. In this work, we compared antifading formulae utilizing a recently developed reducing and oxidizing system (ROXS) with commercial antifading agents. To systematically test fluorophore performance in
Binding of Biotin to Streptavidin: A combined fluorescence correlation spectroscopy and time-resolved fluorescence study.
Stromqvist, J., et al.
The European Physical Journal - Special Topics, 199, 181-194 (2011)

Articles

Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.

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