Stock solutions of Calcein-AM in DMSO are stable at -20 deg. C for up to 12 months when protected from light. Upon dilution to an aqueous working solution (DPBS) it should be used the same day.
56496
Calcein-AM
Small Package (20 X 50 μg ), ≥90.0% (HPLC)
Synonym(s):
Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester, fluorescein complexone, Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester
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About This Item
Empirical Formula (Hill Notation):
C46H46N2O23
CAS Number:
Molecular Weight:
994.86
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Pricing and availability is not currently available.
Quality Segment
assay
≥90.0% (HPLC)
fluorescence
λex 496 nm; λem 516 nm±5 nm in 0.1 M Tris pH 8.0, esterase; Ca2+
storage temp.
−20°C
SMILES string
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
InChI
1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
InChI key
XKFSBWQWNMZWFA-UHFFFAOYSA-N
General description
Calcein-AM, also known as Calcein-acetoxymethyl ester, is a non-fluorescent molecule converted into an anionic fluorescent form by intracellular esterase enzymes. It provides a continuous visualization of adherent cells during the experiment.[1] Calcein-AM is a vital dye that introduces Calcein in living cells with intact cell membranes. The fluorescent part of Calcein-AM localizes in the intracellular spaces after esterase-dependent cellular trapping, displaying cytotoxic activity in cells.[2]
Application
Calcein-AM is used as a cell viability stain and as a neutral substrate for multidrug (MDR) efflux transporters. In cancer research, it is used as a neutral substrate for multidrug resistance protein MRP. Fluorescence assays to determine human erythrocyte viability and aging uses Calcein-AM as a probe.[3]
Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.
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This Item | |||
|---|---|---|---|
| fluorescence λex 496 nm; λem 516 nm±5 nm in 0.1 M Tris pH 8.0, esterase; Ca2+ | fluorescence λex 494 nm, λem 517 nm | fluorescence - | fluorescence λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+ |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| assay ≥90.0% (HPLC) | assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay ≥90% (HPLC) |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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I am wondering how long is Calcein-AM stable in solution approximately ? We dilute Calcein-AM in DPBS 1X supplemented with 1 % HI-FBS. Can we prepare this solution of Calcein-AM diluted in DPBS 1X -1 % HI-FBS the morning to use it the afternoon ? Thanks
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