52412

R-Phycoerythrin

BioReagent, passes test for gel electrophoresis

• Synonym(s): R-PE, phycobiliprotein
• CAS Number: 11016-17-4
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00081950

Properties

• product line: BioReagent
• Quality Segment: 100
• concentration: ≥10.0 mg/mL (UV)
• technique(s): electrophoresis: suitable
• fluorescence: λ_ex 542 nm; λ_em 575 nm in 0.1 M phosphate pH 7.2
• suitability: passes test for gel electrophoresis
• storage temp.: 2-8°C

Description

General description

R-Phycoerythrin or R-PE is a fluorescent probe belonging to the group of phycobiliproteins obtained from macroalgae. R-Phycoerythrin (R-PE) is a protein acting as a photosynthetic accessory pigment in red algae (Rhodophyta). It is an oligomeric protein of 240 kDa, with 6 α (about 20 kDa), 6 β (about 20 kDa), and 1 γ (about 30 kDa) subunits. Phycobiliproteins are water-soluble light-harvesting proteins with a high fluorescent property.

Application

R-Phycoerythrin (R-PE) is useful in the laboratory as a fluorescence-based indicator for the presence of cyanobacteria and a variety of immunofluorescence applications. R-PE is a useful fluoroprobe in various electrophoretic procedures. R-Phycoerythrin (R-PE) proteins detect mercury ions in soil and groundwater samples. Phycoerythrin has been widely used in food, cosmetics, immunodiagnostics, and analytical reagents.

Analysis Note

The product is suspended in 150 mM sodium phosphate, 60% ammonium sulfate, 1 mM EDTA, 1 mM sodium azide, pH 7.0 and must be dialyzed against conjugation buffer or PBS before conjugation.A₅₆₆/A₂₈₀ >4.6, A₆₂₀/A₅₆₆ <0.03, A₅₆₆/A₄₉₈ <1.5

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves