45125

Elastase from porcine pancreas

lyophilized, powder, white, ~8 U/mg

• Synonym(s): Elastase from hog pancreas, Pancreatopeptidase E
• CAS Number: 39445-21-1
• UNSPSC Code: 12352204
• EC Number: 254-453-6
• MDL number: MFCD00130998
• EC Number: 3.4.21.36 (BRENDA, IUBMB)
• Specific activity: ~8 U/mg
• Biological source: Porcine pancreas

Properties

• biological source: Porcine pancreas
• Quality Segment: 100
• form: powder
• quality: lyophilized
• specific activity: ~8 U/mg
• mol wt: M_r ~25000
• impurities: salt, none detected
• color: white
• storage temp.: −20°C

Description

Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Other Notes

For protein sequence studies, limited proteolysis; Application in (selective) hydrolysis/condensation of carboxylic ester bonds

1 U corresponds to the amount of enzyme which liberates 1 μmol 4-nitroaniline per minute at 25°C and pH 7.8 (succinyl-(L-Ala)₃-4-nitroanilide as substrate)

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Sales restrictions may apply

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Codes: H315,H319,H334,H335
• Precautionary Statements: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): dust mask type N95 (US), Eyeshields, Faceshields, Gloves