25455

4-Chloro-7-nitrobenzofurazan

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

• Synonym(s): 4-Chloro-7-nitro-1,2,3-benzoxadiazole, NBD-chloride
• Empirical Formula (Hill Notation): C₆H₂ClN₃O₃
• CAS Number: 10199-89-0
• Molecular Weight: 199.55
• UNSPSC Code: 12352100
• NACRES: NA.32
• PubChem Substance ID: 329752738
• EC Number: 233-496-4
• Beilstein/REAXYS Number: 614212
• MDL number: MFCD00005808
• Assay: ≥97.0% (HPLC)

Properties

• product line: BioReagent
• Quality Segment: 100
• assay: ≥97.0% (HPLC)
• mp: 97-100 °C, 97-99 °C (lit.)
• fluorescence: λ_ex 336 nm in methanol, λ_ex 420 nm; λ_em ~540 nm in ethanol (after derivatization with glycine)
• suitability: suitable for fluorescence
• SMILES string: [O-][N+](=O)c1ccc(Cl)c2nonc12
• InChI: 1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
• InChI key: IGHBXJSNZCFXNK-UHFFFAOYSA-N

Description

Application

4-Chloro-7-nitrobenzofurazan (NBD-chloride) is a fluorescence assay reagent used to label free sulfhydryls and N-terminals within proteins. NBD-chloride is used as an enzyme inhibitor and as a trapping agent for cysteine sulfenic acid.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319
• pcodes: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves