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Sigma-Aldrich

Atto 680-Streptavidin

BioReagent, suitable for fluorescence

Synonym(s):

Streptavidin-Atto 680

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About This Item

MDL number:
UNSPSC Code:
12352108
NACRES:
NA.32

product line

BioReagent

form

powder

manufacturer/tradename

ATTO-TEC GmbH

solubility

H2O: soluble (readily soluble)

suitability

corresponds for gel electrophoresis
suitable for fluorescence

storage temp.

−20°C

Application

Atto 680 is a fluorescent label with high molecular absorption (125.0) and quantum yield (0.30), as well as a sufficient Stoke′s shift (excitation maximum 680 nm, emission maximum 700 nm). It has limited cis-trans-isomerization, which enhances its brightness and reproducibility. Conjugation of Atto 680 to steptavidin does not significantly alter the label′s spectral data regarding excitation and emission maxima. Additionally, the molar absorbance and quantum yield remain high. Such conjugation is useful as a secondary detection reagent in histochemical applications, flow cytometry, microarrays, blot analysis, and immunoassays. Atto 680-streptavin does not show strong fluorescence until bound to biotin.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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