01818

Acylase I from Aspergillus melleus

powder, brown, >0.5 U/mg

• Synonym(s): Aminoacylase, Acylase Amano
• CAS Number: 9012-37-7
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-732-3
• MDL number: MFCD00081285
• EC Number: 3.5.1.14 (BRENDA, IUBMB)
• Specific activity: >0.5 U/mg

Properties

• form: powder
• Quality Segment: 100
• specific activity: >0.5 U/mg
• color: brown
• storage temp.: 2-8°C

Description

General description

Acylase I belongs to the aminoacylase family of enzymes.

Application

Acylase I from Aspergillus melleus has been used to catalyze the Mannich reaction.

Biochem/physiol Actions

Acylase I plays an important role in the amino acid metabolism of organisms. It also plays a role in the acylation of primary and secondary alcohols. Acylase I is involved in alcoholysis.

Analysis Note

Enzyme activity: the optimum temperature is 40-45°C, the optimum pH is 8.0 (stable form pH 6-10). The enzyme is activated by CoCl₂ in the range of 10^-4 to 10^-3 M.

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-acetyl-L-methionine per minute at pH 8.0 and 37°C

Resolution of acetyl amino acids

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)