B0149

Brilliant Blue R

Dye content ~50 %, Powder

• Synonym(s): Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R
• Empirical Formula (Hill Notation): C₄₅H₄₄N₃NaO₇S₂
• CAS Number: 6104-59-2
• Molecular Weight: 825.97
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24891505
• EC Number: 228-060-5
• MDL number: MFCD00041762
• Beilstein/REAXYS Number: 5718025
• Colour Index Number: 42660

Properties

• Product Name: Brilliant Blue R, Dye content ~50 %, Technical grade
• Quality Segment: 200
• form: powder
• composition: Dye content, ~50%
• technique(s): microbe id | staining: suitable
• solubility: H₂O: 10 mg/mL
• suitability: suitable for detection of protein bands following electrophoresis.
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
• InChI: 1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
• InChI key: NKLPQNGYXWVELD-UHFFFAOYSA-M

Description

General description

Brilliant Blue R (C.l. 42660) is a triaminotriarylmethane dye with two amino groups being benzylated and one phenylated. It is also known as brilliant indocyanine 6B and coomassie blue R250.

Application

Brilliant Blue R has been widely used for protein band detection after SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).Its other applications include:

• as a protein stain on epoxy-embedded tissue sections
• for quantitative micro densitometry of protein in sections
• for staining chromatids, actinin microfilaments, developing neurites, and sperm acrosomes.

For detection of protein bands following electrophoresis.

Legal Information

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)