10108987001

Pyrophosphatase, inorganic (PPase)

from yeast

• Synonym(s): inorganic pyrophosphatase
• Enzyme Commission number: 3.6.1.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: yeast
• Quality Level: 100
• form: suspension
• specific activity: ~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.)
• packaging: pkg of 1 mL (1 mg)
• manufacturer/tradename: Roche
• storage temp.: 2-8°C

Description

General description

Pyrophosphate phosphohydrolase

The enzyme pyrophosphatase is ubiquitously distributed in a number of organisms.

Specificity

Specific activity: Approximately 200 U/mg at +25°C with inorganic pyrophosphate as the substrate.

Application

Inorganic pyrophosphatase has been used in enzyme assays and enzyme inhibition studies.

Pyrophosphatase, inorganic (PPase) has been used in :

• Enzymatic nitric oxide (NO) detection system
• In vitro transcription
• Multiply-primed rolling circle amplification

Biochem/physiol Actions

Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate.

Mg ions are necessary for catalytic activity of crystalline pyrophosphatase.

Quality

Contaminants: <0.01% ATPase and phosphatases, each (at pH 7 and 4-nitrophenyl phosphate as the substrate).

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): No data available
• Flash Point(C): No data available