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S7105

Sigma-Aldrich

ApopTag Reaction Buffer

ApopTag Reaction Buffer is intended for use in labeling the 3′-OH ends of fragmented DNA during apoptotic cell detection.

Synonym(s):

ApopTag Buffer, Reaction Buffer

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

Quality Level

manufacturer/tradename

ApopTag
Chemicon®

technique(s)

activity assay: suitable (apoptosis)

shipped in

dry ice

General description

ApopTag Reaction Buffer is intended for use in labeling the 3′-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag In Situ Apoptosis Detection Kits (Catalog #s: S7100, S7101, S7111 and S7165).



Materials Provided

Each vial contains 1 ml of ApopTag Reaction Buffer.



ApopTag is a registered trademark of Serologicals Company.

Application

Materials Provided

Each vial contains 1 ml of ApopTag Reaction Buffer.



Warnings and Precautions

ApopTag Reaction Buffer contains potassium cacodylate (dimethylarsinic acid) as a buffer. This material is harmful if swallowed; avoid contact with skin and eyes (wear gloves, glasses); wash areas of contact immediately.



Instructions for Use

Prepare labeling mixture as follows:

77 μl ApopTag Reaction Buffer

33 μl ApopTag TdT Enzyme (S7107 or kit component # 03)



Mix well and keep on ice. This reagent may be prepared in advance and stored on ice for no more than 6 hours. The Reaction Buffer - TdT Enzyme mixture is ready for use at the "APPLY WORKING STRENGTH TdT ENZYME" step in The Complete ApopTag Manual.

Storage and Stability

Recommended Storage -15°C to -25°C

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Irrit. 2 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3


Certificates of Analysis (COA)

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