The fomulation of this medium is as follows:
Component mM
NaCl 94.62
KCl 4.78
KH2PO4 1.19
MgSO4-7H2O 1.19
CaCl-2H2O 1.71
NaHCO3 4.15
HEPES 20.85
D-Glucose 5.56
Sodium Lactate 23.28
Sodium Pyruvate 0.33
Bovine Serum Albumin 4.0 g/L
Penicillin G Sodium 100 U/mL
Streptomycin 0.05 g/L
Phenol Red 0.01 g/L
MR-015
M2 medium
EmbryoMax M2 Medium (1X), Liquid, with Phenol Red
Synonym(s):
M2 Embryo Media
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.71
eCl@ss:
32160801
Pricing and availability is not currently available.
Quality Segment
form
liquid
manufacturer/tradename
Specialty Media, EmbryoMax®
technique(s)
cell culture | embryo: suitable, cell culture | stem cell: suitable
input
sample type: mouse embryo(s)
Application
EmbryoMax M2 Medium (1X), Liquid, with Phenol Red
Preparation Note
Can be stored at -20°C up to the expiration date on the bottle. Once thawed it should be used within 2 weeks.
Frozen media can be placed in a refrigerator (2°C to 8°C) to thaw overnight. The thawed media should be swirled gently to mix the media, and warmed to 37°C prior to use. Altematively, place frozen medium into a 37°C water bath until thawed completely. It is best to gently swirl the medium during thawing to accelerate the process, and mix the medium. Do not allow the thawed medium to remain in the 37°C water bath for extended periods after it has thawed completely, and warmed to 37°C.
Once thawed the medium has a shelf life of 2 weeks, and must be stored at 2°C to 8°C between uses. Do not re-freeze the medium. Medium stored beyond this 2 week period may not perform optimally.
Note 1:
The "Last Thaw Date" on the label indicates the last day in the shelf life of the frozen media. Media stored frozen beyond this date may not perform optimally. Media are best used prior to the "Last Thaw Date" however, once the "Last Thaw Date" has been reached, the media should be thawed, stored at 2°C to 8°C, and used within 2 weeks.
Note 2:
Although the pH of these media are correct when thawed, the pH of the sodium bicarbonate buffered formulations will rise as the media are exposed to air. This rise in pH will increase with the frequency of opening the bottle, and is particularly rapid when drops of medium placed on a dish for embryo culture are exposed to air. Thus, it is recommended that the drops of medium (placed under light mineral oil) are pre-equilibrated 6 hours to overnight in a humidified incubator at 37°C, in a 5% C02 + 95% Air atmosphere, prior to introducing embryos into the medium.
Frozen media can be placed in a refrigerator (2°C to 8°C) to thaw overnight. The thawed media should be swirled gently to mix the media, and warmed to 37°C prior to use. Altematively, place frozen medium into a 37°C water bath until thawed completely. It is best to gently swirl the medium during thawing to accelerate the process, and mix the medium. Do not allow the thawed medium to remain in the 37°C water bath for extended periods after it has thawed completely, and warmed to 37°C.
Once thawed the medium has a shelf life of 2 weeks, and must be stored at 2°C to 8°C between uses. Do not re-freeze the medium. Medium stored beyond this 2 week period may not perform optimally.
Note 1:
The "Last Thaw Date" on the label indicates the last day in the shelf life of the frozen media. Media stored frozen beyond this date may not perform optimally. Media are best used prior to the "Last Thaw Date" however, once the "Last Thaw Date" has been reached, the media should be thawed, stored at 2°C to 8°C, and used within 2 weeks.
Note 2:
Although the pH of these media are correct when thawed, the pH of the sodium bicarbonate buffered formulations will rise as the media are exposed to air. This rise in pH will increase with the frequency of opening the bottle, and is particularly rapid when drops of medium placed on a dish for embryo culture are exposed to air. Thus, it is recommended that the drops of medium (placed under light mineral oil) are pre-equilibrated 6 hours to overnight in a humidified incubator at 37°C, in a 5% C02 + 95% Air atmosphere, prior to introducing embryos into the medium.
Legal Information
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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What is the full product formulation for the MR-015 formulation of M2 medium?
1 answer-
Helpful?
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Does the MR-015 formulation of M2 medium contain BSA and what concentration?
1 answer-
Yes, this product contains Bovine Serum Albumin at a concentration of 4.0 g/L, equivalent to 0.4% w/v.
Helpful?
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Does the MR-015 formulation of M2 medium contain Bovine Serum Albumin, if so what tissue the concentration?
1 answer-
Yes, this product contains Bovine Serum Albumin at a concentration of 4.0 g/L, equivalent to 0.4% w/v.
Helpful?
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Does the MR-015 formulation of M2 medium contain antibiotics?
1 answer-
The MR-015 formulation of M2 medium does contain antibiotics.
Helpful?
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