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Key Documents

MABC127

Sigma-Aldrich

Anti-Ephrin-B2 Antibody

clone 10H1.1, from mouse

Synonym(s):

Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, LERK-5, HTK ligand, HTK-L

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10H1.1, monoclonal

species reactivity

mouse, rat, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgMκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... EFNB2(1948)

General description

Ephrin-B2 is also called EPH-related receptor tyrosine kinase ligand 5 (LERK-5) and HTK ligand (HTK-L). Ephrin-B2 is a cell surface transmembrane ligand for the Eph tyrosine kinase family of receptors. Ephrin-B2 binds Eph receptors on adjacent cells, leading to contact-dependent bidirectional signaling, which is critical for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Ephrin-B2 is expressed in lung and kidney and together with EPHB4 is involved in heart morphogenesis and angiogenesis.

Immunogen

KLH-conjugated linear peptide corresponding to human Ephrin-B2.

Application

Anti-Ephrin-B2, clone 10H1.1, Cat. No. MABC127, is a mouse monoclonal antibody that detcets Ephrin-B2 and has been tested for use in immunohitochemistry and Western Blotting.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Ephrin-B2 in rat and human lung tissue lysate.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Ephrin-B2 in IHC.

Quality

Evaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ephrin-B2 in 10 µg of mouse lung tissue lysate.

Target description

~65 kDa observed. The calculated molecular weight of this protein is ~37 kDa; however, this protein has been observed at 65-70 kDa (Attwood, B. K., et al. (2011). Nature. 473(7347):372–375.; Lin, K. T., et al. (2008). J Biol Chem. 283(43):28969-28979.).

Physical form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse lung tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nadja Kemmerling et al.
Glia, 65(7), 1103-1118 (2017-04-04)
The Eph-ephrin system plays pivotal roles in cell adhesion and migration. The receptor-like functions of the ephrin ligands allow the regulation of intracellular processes via reverse signaling. γ-Secretase mediated processing of ephrin-B has previously been linked to activation of Src
Ling-Dan Dong et al.
Oncology letters, 13(4), 2109-2114 (2017-04-30)
Endometrial adenocarcinoma (EC) is one of the most frequently diagnosed types of endometrial cancer and is typically a consequence of continuous estrogen receptor stimulation. Erythropoietin-producing hepatocyte receptor B4 (EphB4) and its ligand ephrin-B2 have been reported to be overexpressed in

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